Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-250.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 250 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Research Products International Corp ADA 100 G
- Substance Name: [N-(Carbamoylmethyl) iminodiacetic acid)] [N-(2-Aceta-mido)iminodiacetic acid]
- CAS Number: 26239-55-4
- Molecular Formula: C6H10N2O5
- Molecular Weight: 190.2
![Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00116527-BIOTANGBIOLOGICAL-BUFFERS.JPG-250.jpg)
Biotang Inc MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm
MES [2-(N-Morpholino)-ethanesulfonic acid], 250 gm.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration25KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 100G Sto
Hampton Research StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt 99 titrati25G
MES sodium salt is commonly employed as buffer. Useful pH range for MES sodium salt is 5.5 to 6.7.
Bioworld ACES, 250 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.4) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Bioworld BES, 1M, pH 7.0, 100 mL
BES bufferAlso known as N,N-Bis(hydroxyethyl)-2aminoethanesulfonic acid.Technical Information:pKA (25 C): 7.1Heavy Metals: <0.0005%. Appearance: LiquidBoiling Point : 404.34 °C (Predicted)Density : 1.44 g/cm3 (Predicted)Melting Point: 152-154 °CPH Value: 6.4-7.8
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified | 7365-45-9 | MFCD00006158 | 25g
HEPES BioPerformance Certified | 99.5% | 238.3 | 7365-45-9 | MFCD00006158 | 25g
